zeo dest (Addgene inc)
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zeo dest
Zeo Dest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zeo+dest/pLenti+CMV%2FTO+Zeo+DEST+(644-1)+(Plasmid+%2317294)/pmc12999485-274-13-16
Average 92 stars, based on 7 article reviews
Zeo Dest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zeo+dest/pLenti+CMV%2FTO+Zeo+DEST+(644-1)+(Plasmid+%2317294)/pmc12999485-274-13-16
Average 92 stars, based on 7 article reviews
zeo dest - by Bioz Stars,
2026-09
92/100 stars
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Sequencing:Article Title: A persistent invasive phenotype in post-hypoxic tumor cells is revealed by fate mapping and computational modeling Article Snippet: The loxp-DsRed-loxp-eGFP sequence was amplified by polymerase chain reaction (PCR) from the plasmid pMSCV-loxp-DsRed-loxp- eGFP-Puro-WPRE (#32702, Addgene). .. This sequence was then ligated into the pENTRA1 vector, and the Gateway System TM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Article Title: A persistent invasive phenotype in post-hypoxic tumor cells is revealed by novel fate-mapping and computational modeling Article Snippet: Briefly, the loxp-DsRed-loxp-eGFP sequence was PCR amplified from the plasmid pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (#32702, Addgene). .. This sequence was then ligated into the pENTRA1 vector and the Gateway System TM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Article Title: A Persistent Invasive Phenotype in Post-Hypoxic Tumor Cells is Revealed by Novel Fate-Mapping and Computational Modeling Article Snippet: Briefly, the loxp-DsRed-loxp-eGFP sequence was PCR amplified from the plasmid pMSCV-loxp-DsRedloxp-eGFP-Puro-WPRE (#32702, Addgene). .. This sequence was then ligated into the pENTRA1 vector and the Gateway System TM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Plasmid Preparation:Article Title: A persistent invasive phenotype in post-hypoxic tumor cells is revealed by fate mapping and computational modeling Article Snippet: The loxp-DsRed-loxp-eGFP sequence was amplified by polymerase chain reaction (PCR) from the plasmid pMSCV-loxp-DsRed-loxp- eGFP-Puro-WPRE (#32702, Addgene). .. This sequence was then ligated into the pENTRA1 vector, and the Gateway System TM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Article Title: A persistent invasive phenotype in post-hypoxic tumor cells is revealed by novel fate-mapping and computational modeling Article Snippet: Briefly, the loxp-DsRed-loxp-eGFP sequence was PCR amplified from the plasmid pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (#32702, Addgene). .. This sequence was then ligated into the pENTRA1 vector and the Gateway System TM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: The RNF8KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene, plasmid 98290) virus and selection in medium containing 1 μg ml−1 puromycin. .. RNF8 S-tag rescue lines (described below) were generated by transduction with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: RNF8 KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene plasmid no. 98290) virus and selection in medium containing 1 μg/mL puromycin. .. RNF8 S-tag rescue lines (described in ‘Plasmids and siRNA’) were generated by transduction with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: The RNF8 KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene, plasmid 98290) virus and selection in medium containing 1 μg ml −1 puromycin. .. RNF8 S-tag rescue lines (described below) were generated by transduction with pLenti CMV/TO Article Title: A Persistent Invasive Phenotype in Post-Hypoxic Tumor Cells is Revealed by Novel Fate-Mapping and Computational Modeling Article Snippet: Briefly, the loxp-DsRed-loxp-eGFP sequence was PCR amplified from the plasmid pMSCV-loxp-DsRedloxp-eGFP-Puro-WPRE (#32702, Addgene). .. This sequence was then ligated into the pENTRA1 vector and the Gateway System TM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Stable Transfection:Article Title: Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy. Article Snippet: 100 μg/ml cycloheximide (C4859, Sigma-Aldrich) was added to the cells directly before measurement and luminescence was measured once per hour using a Tecan Spark plate reader. .. Generation of eGFP-expressing cell line For the generation of a stable cell line expressing eGFP under an inducible promoter, the corresponding gene, containing flanking attB sites, was ordered at Twist Bioscience and cloned into pLenti CMV/TO Article Title: Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy Article Snippet: 100 μg/ml cycloheximide (C4859, Sigma-Aldrich) was added to the cells directly before measurement and luminescence was measured once per hour using a Tecan Spark plate reader. .. For the generation of a stable cell line expressing eGFP under an inducible promoter, the corresponding gene, containing flanking attB sites, was ordered at Twist Bioscience and cloned into pLenti CMV/TO Expressing:Article Title: Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy. Article Snippet: 100 μg/ml cycloheximide (C4859, Sigma-Aldrich) was added to the cells directly before measurement and luminescence was measured once per hour using a Tecan Spark plate reader. .. Generation of eGFP-expressing cell line For the generation of a stable cell line expressing eGFP under an inducible promoter, the corresponding gene, containing flanking attB sites, was ordered at Twist Bioscience and cloned into pLenti CMV/TO Article Title: Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy Article Snippet: 100 μg/ml cycloheximide (C4859, Sigma-Aldrich) was added to the cells directly before measurement and luminescence was measured once per hour using a Tecan Spark plate reader. .. For the generation of a stable cell line expressing eGFP under an inducible promoter, the corresponding gene, containing flanking attB sites, was ordered at Twist Bioscience and cloned into pLenti CMV/TO Clone Assay:Article Title: Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy. Article Snippet: 100 μg/ml cycloheximide (C4859, Sigma-Aldrich) was added to the cells directly before measurement and luminescence was measured once per hour using a Tecan Spark plate reader. .. Generation of eGFP-expressing cell line For the generation of a stable cell line expressing eGFP under an inducible promoter, the corresponding gene, containing flanking attB sites, was ordered at Twist Bioscience and cloned into pLenti CMV/TO Article Title: Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy Article Snippet: 100 μg/ml cycloheximide (C4859, Sigma-Aldrich) was added to the cells directly before measurement and luminescence was measured once per hour using a Tecan Spark plate reader. .. For the generation of a stable cell line expressing eGFP under an inducible promoter, the corresponding gene, containing flanking attB sites, was ordered at Twist Bioscience and cloned into pLenti CMV/TO Cloning:Article Title: Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy. Article Snippet: 100 μg/ml cycloheximide (C4859, Sigma-Aldrich) was added to the cells directly before measurement and luminescence was measured once per hour using a Tecan Spark plate reader. .. Generation of eGFP-expressing cell line For the generation of a stable cell line expressing eGFP under an inducible promoter, the corresponding gene, containing flanking attB sites, was ordered at Twist Bioscience and cloned into pLenti CMV/TO Article Title: Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy Article Snippet: 100 μg/ml cycloheximide (C4859, Sigma-Aldrich) was added to the cells directly before measurement and luminescence was measured once per hour using a Tecan Spark plate reader. .. For the generation of a stable cell line expressing eGFP under an inducible promoter, the corresponding gene, containing flanking attB sites, was ordered at Twist Bioscience and cloned into pLenti CMV/TO Generated:Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: The RNF8KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene, plasmid 98290) virus and selection in medium containing 1 μg ml−1 puromycin. .. RNF8 S-tag rescue lines (described below) were generated by transduction with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: RNF8 KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene plasmid no. 98290) virus and selection in medium containing 1 μg/mL puromycin. .. RNF8 S-tag rescue lines (described in ‘Plasmids and siRNA’) were generated by transduction with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: The RNF8 KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene, plasmid 98290) virus and selection in medium containing 1 μg ml −1 puromycin. .. RNF8 S-tag rescue lines (described below) were generated by transduction with pLenti CMV/TO Transduction:Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: The RNF8KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene, plasmid 98290) virus and selection in medium containing 1 μg ml−1 puromycin. .. RNF8 S-tag rescue lines (described below) were generated by transduction with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: RNF8 KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene plasmid no. 98290) virus and selection in medium containing 1 μg/mL puromycin. .. RNF8 S-tag rescue lines (described in ‘Plasmids and siRNA’) were generated by transduction with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: The RNF8 KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene, plasmid 98290) virus and selection in medium containing 1 μg ml −1 puromycin. .. RNF8 S-tag rescue lines (described below) were generated by transduction with pLenti CMV/TO Virus:Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis. Article Snippet: The RNF8KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene, plasmid 98290) virus and selection in medium containing 1 μg ml−1 puromycin. .. RNF8 S-tag rescue lines (described below) were generated by transduction with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: RNF8 KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene plasmid no. 98290) virus and selection in medium containing 1 μg/mL puromycin. .. RNF8 S-tag rescue lines (described in ‘Plasmids and siRNA’) were generated by transduction with pLenti CMV/TO Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis Article Snippet: The RNF8 KO cell line was generated by transduction with lentiCRISPRv2 puro (Addgene, plasmid 98290) virus and selection in medium containing 1 μg ml −1 puromycin. .. RNF8 S-tag rescue lines (described below) were generated by transduction with pLenti CMV/TO |